Publication Date

2015

Journal or Book Title

Journal of the Mechanical Behavior of Biomedical Materials

Abstract

The current knowledge of bone marrow mechanics is limited to its viscous properties, neglecting the elastic contribution of the extracellular matrix. To get a more complete view of the mechanics of marrow, we characterized intact yellow porcine bone marrow using three different, but complementary techniques: rheology, indentation, and cavitation. Our analysis shows that bone marrow is elastic, and has a large amount of intra- and inter-sample heterogeneity, with an effective Young’s modulus ranging from 0.25-24.7 kPa at physiological temperature. Each testing method was consistent across matched tissue samples, and each provided unique benefits depending on user needs. We recommend bulk rheology to capture the effects of temperature on tissue elasticity and moduli, indentation for quantifying local tissue heterogeneity, and cavitation rheology for mitigating destructive sample preparation. We anticipate the knowledge of bone marrow elastic properties for building in vitro models will elucidate mechanisms involved in disease progression and regenerative medicine

Volume

50

Pages

299-307

Funder

SRP was supported by a faculty development award from Barry and Afsaneh Siadat. This work was funded by an NIH New Innovator award (1DP2CA186573-01) awarded to SRP, a grant from the NSF to AJC (DMR-1304724), and start-up funds from the University of Massachusetts Amherst. NPB and JDS thank the James M. Douglas Career Development Faculty Award for support.

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