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Droplet-Interface Bilayer Technologies for Membrane Protein Analysis and Molecular Trafficking Measurements

Max J Lein, University of Massachusetts Amherst

Abstract

Droplet-interface bilayers (DIBs) have been developed as a miniature model membrane system with distinct advantages over cell-based and other artificial membrane systems. This dissertation will describe a number of projects relating to DIB research, including: (i) the creation of new devices that allow the repeated addition and mixing of reagents to one side of a droplet-based membrane system; (ii) measurements of peptide and polymer mediated protein translocation across lipid bilayers; (iii) efforts to synthesize eukaryotic ion channels in DIBs; (iv) investigations on the asymmetric lipid dependence of the potassium channel KirBac1.1.^

Subject Area

Chemistry|Inorganic chemistry|Biophysics

Recommended Citation

Lein, Max J, "Droplet-Interface Bilayer Technologies for Membrane Protein Analysis and Molecular Trafficking Measurements" (2013). Doctoral Dissertations Available from Proquest. AAI3603110.
http://scholarworks.umass.edu/dissertations/AAI3603110

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