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Author ORCID Identifier
N/A
AccessType
Open Access Dissertation
Document Type
dissertation
Degree Name
Doctor of Philosophy (PhD)
Degree Program
Chemistry
Year Degree Awarded
2017
Month Degree Awarded
May
First Advisor
Matthew A. Holden
Subject Categories
Biophysics | Chemistry
Abstract
Pep-1 is a promising peptide tool that delivers proteins and peptides into cells with conserved bioactivity. Pep-1 has great potential because of the high efficiency and lack of toxicity. The mechanism of Pep-1-mediated transport is not fully understood. In my thesis, droplet-interface bilayer (DIB) has been used for the mechanistic studies of Pep-1. Here, DIB is developed for different functions such as quantitation of protein translocation, solution exchange to a formed bilayer and simultaneous observation of multiple membranes. Research work on Pep-1 with DIB reveals that the negative charge of the inner membrane leaflet plays a significant role in promoting cargo translocation. Further investigation indicates that the transport efficiency is time-dependent and complex formation of peptide and cargo is required.
DOI
https://doi.org/10.7275/10014292.0
Recommended Citation
Huang, Jing, "Mechanistic Studies of Peptide-Mediated Protein Transport Across Droplet-Interface Bilayers" (2017). Doctoral Dissertations. 1016.
https://doi.org/10.7275/10014292.0
https://scholarworks.umass.edu/dissertations_2/1016