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Author ORCID Identifier
N/A
AccessType
Open Access Dissertation
Document Type
dissertation
Degree Name
Doctor of Philosophy (PhD)
Degree Program
Chemistry
Year Degree Awarded
2017
Month Degree Awarded
September
First Advisor
Igor A. Kaltashov
Subject Categories
Analytical Chemistry
Abstract
Heparin-like glycosaminoglycan (GAG) is a family of polysaccharide involved in variety of physiological processes. They have potentials to interact with a broad range of proteins and many of them hold crucial values in regulation of protein functions. My dissertation addresses the significance and challenges in the field of heparin-mediated studies, with a focus on the questions in biological and analytical aspects, which are largely hindered by the structural heterogeneity and function diversity of heparin. My dissertation reports the efforts I made in the past few years with respect to the development of novel analytical strategies based on a combination of mass spectrometry, ion-mobility, gas-phase chemistry and chromatography, with success in characterizing protein-GAG interacting stoichiometry and deciphering the structural code related to protein-GAG affinity.
DOI
https://doi.org/10.7275/10088451.0
Recommended Citation
Zhao, Yunlong, "Characterization of Highly Heterogeneous Heparin-Protein Complexes Using Novel Mass Spectrometry-Based Approaches" (2017). Doctoral Dissertations. 1143.
https://doi.org/10.7275/10088451.0
https://scholarworks.umass.edu/dissertations_2/1143