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Author ORCID Identifier
https://orcid.org/0000-0001-7293-4214
AccessType
Open Access Dissertation
Document Type
dissertation
Degree Name
Doctor of Philosophy (PhD)
Degree Program
Kinesiology
Year Degree Awarded
2019
Month Degree Awarded
September
First Advisor
Jane Kent
Second Advisor
Mark Miller
Third Advisor
Edward Debold
Fourth Advisor
Alexander Gerson
Subject Categories
Biochemistry | Exercise Physiology | Exercise Science
Abstract
Skeletal muscle oxidative capacity plays a critical role in human health and disease. Although current models of oxidative phosphorylation sufficiently describe skeletal muscle energetics during moderate-intensity contractions, much is still unknown about the mechanisms that control and limit oxidative phosphorylation during high-intensity contractions. In particular, the oxygen cost of force generation is augmented during exercise at workloads above the lactate threshold. Presently, it is unclear whether this augmentation in muscle oxygen consumption is driven by increased rates of oxidative ATP synthesis (ATPOX) or by decreases in the efficiency of ATPOX due to mitochondrial uncoupling. To address this gap, 31P-MRS was used in two separate studies to measure rates of ATPOX at the end of: 1) repeated bouts of supramaximal knee extensions, and 2) each stage of an isotonic stepwise knee extension protocol. In both studies, high-intensity contractions impaired the maximal capacity for ATPOX and reduced the sensitivity of ATPOX to changes in ADP and cytosolic phosphorylation potential. However, high-intensity contractions did not cause rates of ATPOX to increase relative to workload, suggesting that the increased oxygen cost of exercise above the lactate threshold is due to changes in mitochondrial coupling control (i.e., P/O ratio).
DOI
https://doi.org/10.7275/3r7y-r561
Recommended Citation
Bartlett, Miles F., "Mechanisms that Limit Oxidative Phosphorylation during High-Intensity Muscle Contractions In Vivo" (2019). Doctoral Dissertations. 1687.
https://doi.org/10.7275/3r7y-r561
https://scholarworks.umass.edu/dissertations_2/1687
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