Off-campus UMass Amherst users: To download campus access dissertations, please use the following link to log into our proxy server with your UMass Amherst user name and password.
Non-UMass Amherst users: Please talk to your librarian about requesting this dissertation through interlibrary loan.
Dissertations that have an embargo placed on them will not be available to anyone until the embargo expires.
Author ORCID Identifier
https://orcid.org/0000-0001-7542-4105
AccessType
Open Access Dissertation
Document Type
dissertation
Degree Name
Doctor of Philosophy (PhD)
Degree Program
Molecular and Cellular Biology
Year Degree Awarded
2022
Month Degree Awarded
May
First Advisor
Shelly R. Peyton
Second Advisor
Kathleen Arcaro
Third Advisor
Leonid Pobezinsky
Fourth Advisor
Yubing Sun
Subject Categories
Biomaterials | Cancer Biology
Abstract
Accumulation of epigenetic and genetic changes results in oncogenic transformation of epithelial cells. During breast cancer metastasis, while the extracellular matrix (ECM) becomes stiffer, breast cancer cells transmit mechanical forces into intracellular tension and activate signaling pathways influencing growth, migration, and metastasis. Once cancer cells detach from the primary tumor, they intravasate into the vasculature, survive in the circulation, extravasate and adapt to a new microenvironment of a secondary site. Throughout the process, only a very small population of cancer cells survive, and they are likely to reside at the metastatic sites for several years. The most frequent metastatic sites for breast cancer are brain, lung, liver, and bone. Each has distinct mechanical and biochemical properties. To recapitulate this selection process, we grew triple negative breast cancer (TNBC) on soft and stiff biomaterials in addition to the widely used cell culture platform, tissue culture polystyrene over time. Alterations in phenotypes and gene expressions were captured. Since TNBC is heterogeneous, we were interested in investigating the phenotypes of their subclonal compositions. By isolating subclones from the established TNBC cell lines, we were able to measure variations in growth, motility, response to ECM stiffness and proteins, and drug response, etc.
DOI
https://doi.org/10.7275/28638785
Recommended Citation
Tseng, Ning-Hsuan, "EXTRACELLULAR MATRIX STIFFNESS AS A CUE TO SHAPE PHENOTYPIC EVOLUTION OF TRIPLE NEGATIVE BREAST CANCER" (2022). Doctoral Dissertations. 2577.
https://doi.org/10.7275/28638785
https://scholarworks.umass.edu/dissertations_2/2577