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ORCID
N/A
Access Type
Open Access Thesis
Document Type
thesis
Degree Program
Chemistry
Degree Type
Master of Science (M.S.)
Year Degree Awarded
2015
Month Degree Awarded
September
Abstract
Nanoparticles (NPs) have been used widely in various fields ranging from biomedical applications to life science due to their highly tunable properties. It is essential to understanding how NPs interact with biological systems of interest, therefore, analytical platforms to efficiently track NPs from cell to animal level are essential. In this thesis, laser desorption ionization mass spectrometry (LDI-MS) and inductively-coupled plasma mass spectrometry (ICP-MS) has been developed and applied to quantify NP/cell and NP/biological surface interactions. These two methods provide fast, label-free and quantitative analysis. New capability of LDI-MS to differentiate cell surface-bound and internalized NPs were established and ICP-MS coupled with a library of surface- functionalized AuNPs were used to probe the affinity between NPs and human hair surface. NPs interacting with biological surfaces and plasma membrane were quantified and the interactions were controlled by the chemical properties of the interface between NP and biological systems.
DOI
https://doi.org/10.7275/7463672
First Advisor
Vincent M Rotello
Second Advisor
Richard W Vachet
Recommended Citation
Hou, Singyuk, "Studying Nanoparticle/cell and Nanoparticle/biosurface Interaction with Mass Spectrometry" (2015). Masters Theses. 273.
https://doi.org/10.7275/7463672
https://scholarworks.umass.edu/masters_theses_2/273