Document Type

Open Access Thesis

Embargo Period

8-14-2015

Degree Program

Chemistry

Degree Type

Master of Science (M.S.)

Year Degree Awarded

2015

Month Degree Awarded

September

Advisor Name

Vincent

Advisor Middle Initial

M

Advisor Last Name

Rotello

Co-advisor Name

Richard

Co-advisor Middle Initial

W

Co-advisor Last Name

Vachet

Abstract

Nanoparticles (NPs) have been used widely in various fields ranging from biomedical applications to life science due to their highly tunable properties. It is essential to understanding how NPs interact with biological systems of interest, therefore, analytical platforms to efficiently track NPs from cell to animal level are essential. In this thesis, laser desorption ionization mass spectrometry (LDI-MS) and inductively-coupled plasma mass spectrometry (ICP-MS) has been developed and applied to quantify NP/cell and NP/biological surface interactions. These two methods provide fast, label-free and quantitative analysis. New capability of LDI-MS to differentiate cell surface-bound and internalized NPs were established and ICP-MS coupled with a library of surface- functionalized AuNPs were used to probe the affinity between NPs and human hair surface. NPs interacting with biological surfaces and plasma membrane were quantified and the interactions were controlled by the chemical properties of the interface between NP and biological systems.

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