Document Type

Open Access Thesis

Embargo Period

5-22-2015

Degree Program

Neuroscience & Behavior

Degree Type

Master of Science (M.S.)

Year Degree Awarded

2015

Month Degree Awarded

September

Advisor Name

James

Advisor Middle Initial

J

Advisor Last Name

Chambers

Abstract

Ciprofloxacin is a widely prescribed antibiotic which causes idiopathic sensory adverse effects and is known to induce oxidative stress. Dopaminergic cells are known to have intrinsic sensitivity to oxidative stress. To investigate whether ciprofloxacin potentiates cy- totoxicity of dopamine, effects of combined drug treatments on cell viability were assessed by resazurin reduction, and effects on mitochondrial health were assessed by morphology. The cell viability assays suggest that ciprofloxacin significantly potentiates dopamine cytotoxicity at clinically relevant doses, although dopamine possibly interferes with the viability assay. Effects of drug treatments on mitochondrial morphology were inconclusive.

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