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USE OF FUNCTIONALIZED GOLD NANOPARTICLES TO EFFICIENTLY EXTRACT AND CONCENTRATE PEPTIDES FOR MALDI-TOF-MS DETECTION
Document Type: Open Access
Degree Program
Chemistry
Degree Type
Master of Science (M.S.)
Year Degree Awarded
2007
Month Degree Awarded
May
Primary Subject Category
Analytical chemistry
Advisor(s) or Committee Chair
Vachet, Richard W
Abstract
USE OF FUNCTIONALIZED GOLD NANOPARTICLES TO EFFICIENTLY EXTRACT AND CONCENTRATE PEPTIDES FOR MALDI-TOF-MS DETECTION
MAY 2007
BENJAMIN PAPA KWESI NII YANKOS VANDERPUIJE, B.Sc, KWAME
NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY, GHANA
M.S., UNIVERSITY OF MASSACHUSETTS AMHERST
Directed by: Professor Richard W. Vachet
We have developed a straight forward method that uses monolayer protected clusters (MPCs) and mixed monolayer protected clusters (MMPCs) as selective extraction and concentration probes for peptides. After extraction with these nanoparticles (NPs), the peptides are analyzed by MALDI-TOF-MS to obtain their mass fingerprints. Application of the method to a test library of 146 tryptic peptides showed that cationic MPCs/MMPCs target negatively charged peptides while the anionic MPCs/MMPCs target positively charged peptides. The extraction with these NPs is also accompanied by high concentration factors such they can be used to extract and concentrate microcystin-LR at levels below the WHO guideline of 1μg/L.
Recommended Citation
Vanderpuije, Benjamin P. K. N. Y, "USE OF FUNCTIONALIZED GOLD NANOPARTICLES TO EFFICIENTLY EXTRACT AND CONCENTRATE PEPTIDES FOR MALDI-TOF-MS DETECTION" (2007). Masters Theses. Paper 18.
http://scholarworks.umass.edu/theses/18
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