Emerging public health concerns relating to the epigenetic effects of EDCs, along with the reconceptualization of dose response curves, provides a compelling rationale for addressing estrogenically active contaminants in drinking water. These environmental health concerns are now known to have long lasting impacts, especially on fetal development. For this drinking water research, the estrogenic EDC byproducts were identified and the treatment processes were compared using the dose applied, the number of byproducts formed and the relative quantification of the treatment byproducts. The analytical optimized method presented and implemented in this research successfully determined the percent degradation of the parent compound for each disinfection treatment selected. From the resulting data, the chlorination of EE2 and DES produced the highest percent degradation of the parent compound, with the least number of byproducts. The optimized method decreased sample variability; showed a better fit to a linear calibration with both high and low concentrations of the parent compound; and lower MQLs and MDLs. Continuing research is needed to help in understanding the complete consequences of estrogenic endocrine disruptors in drinking water and the inevitable public health impact.