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Due to its excellent mechanical properties, dense microstructure, low permeability, ease of placement and volume stability, ultra-high performance concrete (UHPC) is considered the next-generation structural concrete and is increasingly used in transportation infrastructure. While previous research efforts generated valuable results, to achieve the desired performance, UHPC needs to be well formulated with precise and optimized quantities of cementitious materials, fillers, fine aggregate, water, chemical admixtures, and fibers. In addition, the mixture design of UHPC and its correlation with the performance evolution under different curing conditions remain unclear, and there exist critical significant gaps in understanding the efficiency of fibers and mixture design on the properties of UHPC, especially the mechanical and durability-related performance. Massachusetts Department of Transportation (MassDOT) is exploring multiple infrastructure applications that can incorporate UHPC, including joints, overlays, repairs, rehabilitation, and bridge beam fabrication. This project aims to develop UHPC mix design formulations that can be implemented at ready-mix batching plants or precast/prestressed concrete fabrication facilities by identifying and maximizing the roles of fibers and additives in enhancing mechanical and durability-related properties. Four fiber-reinforced mixes and seven UHPC mixes with different fibers were investigated and a UHPC mix for large-scale batching and field applications was recommended.