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Biofilm infections are a global public health threat, necessitating new treatment strategies. Biofilm formation also contributes to the development and spread of multidrug-resistant (MDR) bacterial strains. Biofilm-associated chronic infections typically involve colonization by more than one bacterial species. The co-existence of multiple species of bacteria in biofilms exacerbates therapeutic challenges and can render traditional antibiotics ineffective. Polymeric nanoparticles offer alternative antimicrobial approaches to antibiotics, owing to their tunable physico-chemical properties. Here, we report the efficacy of poly(oxanorborneneimide) (PONI)-based antimicrobial polymeric nanoparticles (PNPs) against multi-species bacterial biofilms. PNPs showed good dual-species biofilm penetration profiles as confirmed by confocal laser scanning microscopy. Broad-spectrum antimicrobial activity was observed, with reduction in both bacterial viability and overall biofilm mass. Further, PNPs displayed minimal fibroblast toxicity and high antimicrobial activity in an in vitro co-culture model comprising fibroblast cells and dual-species biofilms of Escherichia coli and Pseudomonas aeruginosa. This study highlights a potential clinical application of the presented polymeric platform.
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NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01 AI134770]
Makabenta, Jessa Marie V.; Park, Jungmi; Li, Cheng-Hsuan; Chattopadhyay, Aritra Nath; Nabawy, Ahmed; Landis, Ryan F.; Gupta, Akash; Schmidt-Malan, Suzannah; Patel, Robin; and Rotello, Vincent M., "Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms" (2021). Molecules. 1485.