Off-campus UMass Amherst users: To download dissertations, please use the following link to log into our proxy server with your UMass Amherst user name and password.

Non-UMass Amherst users, please click the view more button below to purchase a copy of this dissertation from Proquest.

(Some titles may also be available free of charge in our Open Access Dissertation Collection, so please check there first.)

From initiation to elongation in T7 RNA polymerase

Edward A Esposito, University of Massachusetts Amherst


The process of transcription is quite complicated and involves many more steps than the classic textbook view of: Bind, melt, transcribe, release. We have studied the complex nature of transcription from the initiation of transcription through the transition from an unstable initiation complex to a stable, processive, elongation complex. We show that promoter release is not required to make the transition to a stable elongation complex. We show conclusively that a proposed model of transient excursion is not adequate for explaining the abortive cycling process that occurs in all known polymerases. We have proposed and tested a model that unites the processes of promoter release, collapse of the initially melted bubble, and proper RNA displacement. Additionally, we have challenged the existing model for the mechanism of inhibition by T7 lysozyme on T7 RNA polymerase.

Subject Area

Biochemistry|Molecular biology

Recommended Citation

Esposito, Edward A, "From initiation to elongation in T7 RNA polymerase" (2006). Doctoral Dissertations Available from Proquest. AAI3206193.