Off-campus UMass Amherst users: To download dissertations, please use the following link to log into our proxy server with your UMass Amherst user name and password.
Non-UMass Amherst users, please click the view more button below to purchase a copy of this dissertation from Proquest.
(Some titles may also be available free of charge in our Open Access Dissertation Collection, so please check there first.)
Interaction of amphiphilic macromolecules with globular proteins
Abstract
The application of bionanotechnology in medicine has tremendously increased over past three decades. The role of synthetic polymer/supramolecular chemistry has proven to be a key component in this multidisciplinary research. Towards that direction, this thesis work describes the entry of our group research in the area of “applications of custom designed amphiphilic macromolecules in medicine or biology”. We have successfully used our amphiphilic homopolymer scaffold to recognize, bind and modulate the function of the proteins. The proof of concept was demonstrated using two proteins, chymotrypsin and cytochrome c. Further, We have also synthesized water-soluble fluorescent organic nanosensors by functionalizing the amphiphilic polymers with appropriate organic dye. These fluorescent nanosensors have been effectively used to detect the presence of metalloproteins in the presence of nonmetalloproteins. Along that direction, we have also utilized our scaffold to detect a particular metalloproteins by creating pattern using Stern-Volmer quenching studies.
Subject Area
Organic chemistry
Recommended Citation
Sandanaraj, Britto S, "Interaction of amphiphilic macromolecules with globular proteins" (2007). Doctoral Dissertations Available from Proquest. AAI3289226.
https://scholarworks.umass.edu/dissertations/AAI3289226