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Surface functionalized nanoparticles for DNA and drug delivery
Abstract
Surface functionized nanoparticles provide versatile tools for biotechnology. Their unique nano-scale size and large surface-volume ratio make them ideal for recognition of small molecules, DNA and proteins. Here, we are using such nanoparticles as DNA/drug delivery agents in mammalian cells. The release processes were mediated inside cells using glutathione (GSH) or near-UV light. Both in vitro and in vivo release of drug/DNA payload from nanoparticle surface were established. Additionally, a systemic study of nanoparticles surface charge relationship of various aspects of drug delivery (e.g. intracellular payload release, cellular uptake, and tissue penetration) has been performed using fluorescein-conjugated model systems. Furthermore, the GSH- or light- mediated release, combining with the controlled interactions of biomolecules with surface functionalized nanoparticle scaffolds, can be further utilized to realize targeted delivery into cancer cells.
Subject Area
Biochemistry|Organic chemistry
Recommended Citation
Han, Gang, "Surface functionalized nanoparticles for DNA and drug delivery" (2007). Doctoral Dissertations Available from Proquest. AAI3289283.
https://scholarworks.umass.edu/dissertations/AAI3289283