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Surface functionalized nanoparticles for DNA and drug delivery
Surface functionized nanoparticles provide versatile tools for biotechnology. Their unique nano-scale size and large surface-volume ratio make them ideal for recognition of small molecules, DNA and proteins. Here, we are using such nanoparticles as DNA/drug delivery agents in mammalian cells. The release processes were mediated inside cells using glutathione (GSH) or near-UV light. Both in vitro and in vivo release of drug/DNA payload from nanoparticle surface were established. Additionally, a systemic study of nanoparticles surface charge relationship of various aspects of drug delivery (e.g. intracellular payload release, cellular uptake, and tissue penetration) has been performed using fluorescein-conjugated model systems. Furthermore, the GSH- or light- mediated release, combining with the controlled interactions of biomolecules with surface functionalized nanoparticle scaffolds, can be further utilized to realize targeted delivery into cancer cells.
Han, Gang, "Surface functionalized nanoparticles for DNA and drug delivery" (2007). Doctoral Dissertations Available from Proquest. AAI3289283.