Off-campus UMass Amherst users: To download campus access dissertations, please use the following link to log into our proxy server with your UMass Amherst user name and password.

Non-UMass Amherst users: Please talk to your librarian about requesting this dissertation through interlibrary loan.

Dissertations that have an embargo placed on them will not be available to anyone until the embargo expires.

Date of Award

5-2012

Document Type

Campus Access

Degree Name

Doctor of Philosophy (PhD)

Degree Program

Plant Biology

First Advisor

Elsbeth L. Walker

Second Advisor

Magdalena Bezanilla

Third Advisor

Samuel P. Hazen

Subject Categories

Genetics | Molecular Biology | Plant Sciences

Abstract

Brachypodium distachyon (brachypodium) is a temperate grass that has great promise as a model system to study grass-specific traits for crop improvement. Under iron (Fe)-deficient conditions, grasses synthesize and secrete Fe(III)-chelating agents called phytosiderophores (PS). In maize, Yellow Stripe1 (ZmYS1) is the transporter responsible for uptake of Fe(III)-PS complexes from the soil. Some members of the family of related proteins called Yellow Stripe-Like (YSL) have roles in internal Fe translocation of plants, while the function of other members remains uninvestigated.

One aim of this study was to establish brachypodium as a model system to study Fe homeostasis in grasses. HDMA was detected as the dominant type of PS secreted by brachypodium, and its secretion is diurnally regulated in parallel to that of related species such as barley and wheat. Nineteen YSL family members were identified in brachypodium, and their expression profiles in response to Fe deficiency were analyzed. Phylogenetic analysis revealed that some YSLs group into a grass-specific clade, and expression of the BdYSLmembers of this clade could not be detected in shoots or roots, suggesting grass-specific functions in reproductive tissues. The Fe status of the plant can regulate expression of several BdYSL genes in both shoots and roots suggesting roles in Fe homeostasis. Brachypodium YS1 knockdown lines were also generated to test Fe(III)-PS transport abilities of certain YSL proteins.

In the context of this dissertation, an EMS mutagenized population of brachypodium was produced. One mutant with the iron deficiency symptom interveinal chlorosis was characterized. Metal content analyses revealed that mutant plants are low in Fe, Zn and Cu. Mutant plants are capable of synthesizing PS in normal amounts, but they do not release the PS from roots into the soil solution, suggesting that gene underlying this mutation functions to allow secretion of PS. Performing bulk segregant array mapping and fine mapping using SNP markers, the location of this new mutation was mapped to a 1.7Mb interval on chromosome 5. Cloning of this gene is likely to fill in the missing part of the iron uptake mechanism in grasses.

Share

COinS