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Date of Award
Doctor of Philosophy (PhD)
Matthew A. Holden
Richard W. Vachet
Biophysics | Chemistry | Inorganic Chemistry
Droplet-interface bilayers (DIBs) have been developed as a miniature model membrane system with distinct advantages over cell-based and other artificial membrane systems. This dissertation will describe a number of projects relating to DIB research, including: (i) the creation of new devices that allow the repeated addition and mixing of reagents to one side of a droplet-based membrane system; (ii) measurements of peptide and polymer mediated protein translocation across lipid bilayers; (iii) efforts to synthesize eukaryotic ion channels in DIBs; (iv) investigations on the asymmetric lipid dependence of the potassium channel KirBac1.1.
Lein, Max J, "Droplet-Interface Bilayer Technologies for Membrane Protein Analysis and Molecular Trafficking Measurements" (2013). Doctoral Dissertations 1896 - February 2014. 546.