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Author ORCID Identifier
N/A
AccessType
Open Access Dissertation
Document Type
dissertation
Degree Name
Doctor of Philosophy (PhD)
Degree Program
Chemistry
Year Degree Awarded
2017
Month Degree Awarded
September
First Advisor
Vincent M Rotello
Subject Categories
Biomedical Engineering and Bioengineering | Biotechnology | Cell Biology | Materials Chemistry
Abstract
Direct cytoplasmic delivery of gene editing nucleases such CRISPR/Cas9 systems and therapeutic proteins provides enormous opportunities in curing human genetic diseases, and assist research in basic cell biology. One approach to attain such a goal is through engineering nanotechnological tools to mimic naturally existing intra- and extracellular protein delivery/transport systems. Nature builds transport systems for proteins and other biomolecules through evolution-derived sophisticated molecular engineering. Inspired by such natural assemblies, I employed molecular engineering approaches to fabricate self-assembled nanostructures to use as intracellular protein delivery tools. Briefly, proteins and gold nanoparticles were co-engineered to carry complementary electrostatic recognition elements. When these materials were mixed together, they formed highly sophisticated, multi-layered, and hierarchical self-assembled nanostructures of few hundred-nanometer size. These structures carried a large number of engineered proteins, got fused to cell membrane upon incubation, and delivered the encapsulated protein content directly into cell cytoplasm. Using this technology, we delivered a wide range of proteins, and CRISPR/Cas9-ribonucleoprotein that resulted high efficient gene editing.
DOI
https://doi.org/10.7275/10275569.0
Recommended Citation
Mout, Rubul, "PROTEIN-NANOPARTICLE CO-ENGINEERING: SELF-ASSEMBLY, INTRACELLULAR PROTEIN DELIVERY, AND CRISPR/CAS9-BASED GENE EDITING" (2017). Doctoral Dissertations. 1112.
https://doi.org/10.7275/10275569.0
https://scholarworks.umass.edu/dissertations_2/1112
Included in
Biomedical Engineering and Bioengineering Commons, Biotechnology Commons, Cell Biology Commons, Materials Chemistry Commons