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Document Type

Open Access Dissertation

Degree Name

Doctor of Philosophy (PhD)

Degree Program

Chemistry

Year Degree Awarded

2017

Month Degree Awarded

September

First Advisor

Igor A. Kaltashov

Subject Categories

Analytical Chemistry

Abstract

Heparin-like glycosaminoglycan (GAG) is a family of polysaccharide involved in variety of physiological processes. They have potentials to interact with a broad range of proteins and many of them hold crucial values in regulation of protein functions. My dissertation addresses the significance and challenges in the field of heparin-mediated studies, with a focus on the questions in biological and analytical aspects, which are largely hindered by the structural heterogeneity and function diversity of heparin. My dissertation reports the efforts I made in the past few years with respect to the development of novel analytical strategies based on a combination of mass spectrometry, ion-mobility, gas-phase chemistry and chromatography, with success in characterizing protein-GAG interacting stoichiometry and deciphering the structural code related to protein-GAG affinity.

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