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Open Access Dissertation
Doctor of Philosophy (PhD)
Year Degree Awarded
Month Degree Awarded
Igor A. Kaltashov
Heparin-like glycosaminoglycan (GAG) is a family of polysaccharide involved in variety of physiological processes. They have potentials to interact with a broad range of proteins and many of them hold crucial values in regulation of protein functions. My dissertation addresses the significance and challenges in the field of heparin-mediated studies, with a focus on the questions in biological and analytical aspects, which are largely hindered by the structural heterogeneity and function diversity of heparin. My dissertation reports the efforts I made in the past few years with respect to the development of novel analytical strategies based on a combination of mass spectrometry, ion-mobility, gas-phase chemistry and chromatography, with success in characterizing protein-GAG interacting stoichiometry and deciphering the structural code related to protein-GAG affinity.
Zhao, Yunlong, "Characterization of Highly Heterogeneous Heparin-Protein Complexes Using Novel Mass Spectrometry-Based Approaches" (2017). Doctoral Dissertations. 1143.