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Author ORCID Identifier

https://orcid.org/0000-0001-7496-0416

Document Type

Open Access Dissertation

Degree Name

Doctor of Philosophy (PhD)

Degree Program

Food Science

Year Degree Awarded

2019

Month Degree Awarded

September

First Advisor

Lili He

Subject Categories

Agriculture | Food Science | Microbiology

Abstract

The growth curves for E. coli O157:H7 (#043888) are reported. We make the case that the onset of stationary growth is the optimal point at which a bacteria culture is considered suitable for quantitative Raman analyses. The optimal conditions for 3-mercaptophenylboronic acid coating of bacteria cells is also reported. Fundamental drawbacks of the status-quo approach have been elucidated and overcome, based on measurable improvements to the experimental methodology. This approach is shown to be suitable for the evaluation of bacterial rinse-washing efficacy by means of Raman light-scattering. The data were compared to label-free applications and the measurable differences between each approach were defined. Future use of 3-mercaptophenylboronic acid labelling for SERS analyses of bacteria should strictly follow the methods that we outlined within this paper. A real-time method to surveil mass bacterial communities directly in situ is also reported. The approach was successfully employed to indiscriminately monitor mass bacteria populations directly among plant tissue.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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