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Author ORCID Identifier

https://orcid.org/0000-0003-3904-592X

AccessType

Open Access Dissertation

Degree Name

Doctor of Philosophy (PhD)

Degree Program

Neuroscience and Behavior

Year Degree Awarded

2020

Month Degree Awarded

September

First Advisor

Eric L. Bittman

Second Advisor

Joseph F. Bergan

Third Advisor

Tanya L. Leise

Fourth Advisor

David R. Weaver

Subject Categories

Endocrinology | Neuroscience and Neurobiology | Physiology

Abstract

Endogenous daily (circadian) oscillators ensure the proper timing of physiological and behavioral processes that are essential to health. A set of core clock genes and their protein products function in transcriptional/translational feedback loops (TTFLs) that time and coordinate vital homeostatic, cognitive, and hormonal processes. A master pacemaker in the suprachiasmatic nucleus (SCN) coordinates circadian function throughout the organism. Investigating the causal role of genetically defined cell types in the suprachiasmatic nucleus for circadian rhythms is essential to understand how an animal’s physiology is temporally organized. This thesis explores the role of molecular clocks within particular cell types in regulating physiology. This characterization spans the spectrum from behavior/physiology at the organismal level to characterizing circadian function at the tissue/cell level. Chapter 1 provides a basic overview of circadian rhythms and how they regulate physiology and sets the stage for subsequent sections. In Chapter 2, I describe experiments we performed using conditional mouse models lacking clock function within a circuit that regulates the Luteinizing Hormone (LH) surge. I showed that circadian function within multiple cell types is necessary for the proper timing of LH surge. In Chapter 3, I report on the efforts to determine whether duper alters autonomous clock mechanism (the TTFL) or whether duper alters intercellular coupling of circadian oscillators within the SCN. In Chapter 4, I extend the characterization of the effects of a novel, spontaneous circadian mutation, duper, on the pre-ovulatory LH surge. I conclude by proposing a model to explain shifting of phase in a multioscillatory circuit.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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