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Author ORCID Identifier
Campus-Only Access for Five (5) Years
Doctor of Philosophy (PhD)
Year Degree Awarded
Month Degree Awarded
M. Sloan Siegrist
Yasu S. Morita
Michele M. Klingbeil
Bacteriology | Microbial Physiology
Bacterial cells are protected by their cell envelope. Diversity in architecture and synthesis mechanisms of the envelope is matched by the diversity in environments inhabited by the bacterial domain. The Mycobacterium genus includes animal and human pathogens, nonpathogenic environmental species, and acts as a model organism for rod-shaped bacteria that grow by adding new material at their polar region. Mycobacteria exhibit many unusual features in both the structure of their envelope as well as how it is assembled. Extensive work in the cell wall field has described how peptidoglycan is organized and polymerized. However, existing models for these processes are unlikely to apply to members of the mycobacterial genus, as they lack key elements of established models. In this dissertation, we use Mycobacterium smegmatis as a model organism to study mycobacterial cell envelope synthesis, rod shape maintenance, and the relationship between the two. We focus on cytoskeletal protein, DivIVA, the architect of polar growth and rod shape, the role of lipid II transglycosylase RodA, response to cell wall damage, and finally, we use single molecule localization microscopy to understand the dynamics of mycobacterial cell envelope assembly.
Melzer, Emily Sarah, "Mycobacterial Morphology and Cell Envelope: Construction and Maintenance" (2021). Doctoral Dissertations. 2255.