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Author ORCID Identifier
N/A
AccessType
Open Access Dissertation
Document Type
dissertation
Degree Name
Doctor of Philosophy (PhD)
Degree Program
Polymer Science and Engineering
Year Degree Awarded
2014
Month Degree Awarded
February
First Advisor
Tew, Gregory N.
Second Advisor
Santore, Maria
Third Advisor
Dinsmore, Anthony
Subject Categories
Biophysics | Polymer Chemistry
Abstract
Protein transduction domains (PTDs) and their and their synthetic mimics are short sequences capable of unusually high uptake in cells. Several varieties of these molecules, including the arginine-rich Tat peptide from HIV, have been extensively used as vectors for protein, DNA, and siRNA delivery into cells. Despite the wide-ranging utility of PTDs and their mimics, their uptake mechanism is still under considerable debate. How the molecules are able to cross phospholipid membranes, and what structural components are necessary for optimal activity are poorly understood. This thesis explores how PTDMs interact with phospholipid membrane phase, anionic lipid content and negative Gaussian curvature generation along the structural variables of polymer length, charge density, hydrophobicity, aromaticity, and architecture. From these it is demonstrated that PTDM-membrane interaction is primarily controlled by balancing solubility of polymer in solution and in the lipid. Barriers to activity, such as anionic lipid content and membrane rigidity, can be overcome by the addition of hydrophobicity, but this has to be balanced by considerations of water solubility and toxicity.
DOI
https://doi.org/10.7275/6041519.0
Recommended Citation
Lis, Michael T W, "Guanidinium-Rich ROMP Polymers Drive Phase, Charge, and Curvature-Specific Interactions with Phospholipid Membranes" (2014). Doctoral Dissertations. 231.
https://doi.org/10.7275/6041519.0
https://scholarworks.umass.edu/dissertations_2/231