Off-campus UMass Amherst users: To download campus access dissertations, please use the following link to log into our proxy server with your UMass Amherst user name and password.

Non-UMass Amherst users: Please talk to your librarian about requesting this dissertation through interlibrary loan.

Dissertations that have an embargo placed on them will not be available to anyone until the embargo expires.

Author ORCID Identifier

https://orcid.org/0000-0001-6968-03210000-0001-6968-0321

AccessType

Open Access Dissertation

Document Type

dissertation

Degree Name

Doctor of Philosophy (PhD)

Degree Program

Animal Biotechnology & Biomedical Sciences

Year Degree Awarded

2022

Month Degree Awarded

February

First Advisor

Janice C. Telfer

Second Advisor

Cynthia L. Baldwin

Subject Categories

Animal Sciences | Genomics | Immunology and Infectious Disease

Abstract

gd T cells are a crucial component of the immune response to a number of increasingly relevant and largely zoonotic pathogens to which efficacious vaccination is lacking. In ruminants and swine, gd T cells represent a major population of peripheral blood and epithelial tissue-resident lymphocytes. gdT cells respond to both protein and non-protein antigens independently of MHC presentation and possess immunological memory. Upon activation, gamma delta T cells illicit a variety of effector functions and play an indispensable role of orchestrating the downstream immune response. These characteristics make gamma delta T cells a promising candidate for recruitment by vaccination, however, methods for effectively priming these cells remain to be elucidated. The type I transmembrane receptor Workshop Cluster One (WC1) is expressed as a multigenic array on gd T cells in swine and ruminants. In cattle there are 13 unique WC1 genes (WC1-1 to WC1-13) each comprised of 6-11 SRCR domains that selectively bind unprocessed antigen in a manner that resembles a pattern recognition receptor (PRRs). WC1 functions as a hybrid PRR and co-receptor for the gamma delta TCR as it potentiates activation signals from the TCR and dictates antigen specificity of expressing gd T cells. cDNA evidence suggests that porcine WC1 is expressed as a multigenic array consisting of 9 genes (WC1-1 to WC1-9) each encoding 6 SRCR domains with unique pathogen binding potential. The objective of this study is to characterize the multigenic array of porcine WC1, investigate its propensity for pathogen binding, and evaluate its expression on gd T cells. Using the MAKER annotation pipeline, we annotated Sscrofa11.1 for sequence derived from full-length cDNA transcripts representing the 9 porcine WC1 genes. We were able to map 8 of the 9 genes, leaving one (WC1-8) unplaced in the current assembly. We defined three subpopulations of porcine gd T cells based on expression of WC1 and CD2. Finally, we confirmed that porcine WC1 SRCR domains are capable of directly binding whole fixed bacteria including Leptospira spp and Mycobacterium bovis.

DOI

https://doi.org/10.7275/27230449

Share

COinS