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Author ORCID Identifier

N/A

AccessType

Open Access Dissertation

Document Type

dissertation

Degree Name

Doctor of Philosophy (PhD)

Degree Program

Chemistry

Year Degree Awarded

2017

Month Degree Awarded

February

First Advisor

RICHARD W. VACHET

Second Advisor

EDWARD G. VOIGTMAN

Third Advisor

MICHAEL J. KNAPP

Fourth Advisor

BAOSHAN XING

Subject Categories

Analytical Chemistry | Chemistry | Physical Sciences and Mathematics

Abstract

Their smaller size, inherent non-toxicity and tunable properties of gold nanoparticles (AuNPs) attract researchers for their use in biological applications such as drug delivery, imaging and therapeutics. Understanding the in vivo fate of these AuNPs are essential for their potential effects in both the environment and the body. In this dissertation, mass spectrometric imaging methods using laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS) and laser desorption/ionization (LDI-MS) have been investigated to monitor the in vivo fate of AuNPs. AuNP injected mouse tissue samples can be obtained and readily imaged to track the injected AuNPs using these methods. A first-ever imaging of 2 nm monolayer protected AuNPs in vivo using LA-ICP-MS is described. In addition, sub-organ biodistribution of AuNPs using LA-ICP-MS has been investigated. An alternative quantification strategy that can be used for LA-ICP-MS is expressed. A dual mode imaging method that can be used to monitor the stability of AuNPs by combining LA-ICP-MS and LDI-MS is reported.

DOI

https://doi.org/10.7275/9203049.0

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