Spawning and rearing habitats used by white sturgeons (Acipenser transmontanus)were described from water temperature, depth, and velocity measurements and substrate typespresent at sites where eggs, larvae, young-of-the-year, and juveniles (ages 1-7) were collected.Spawning and egg incubation occurred in the swiftest water available (mean water columnvelocity, 0.8-2.8 m/s), which was within 8 km downstream from each of the four main-stem Columbia River dams in our study area. Substrates where spawning occurred were mainlycobble, boulder, and bedrock. Yolk-sac larvae were transported by the river currents fromspawning areas into deeper areas with lower water velocities and finer substrates. Young-of-theyearwhite sturgeons were found at depths of 9–57 m, at mean water column velocities of 0.6 m/sand less, and over substrates of hard clay, mud and silt, sand, gravel, and cobble. Juvenile fishwere found at depths of 2–58 m, at mean water column velocities of 1.2 m/s and less, and oversubstrates of hard clay, mud and silt, sand, gravel, cobble, boulder, and bedrock.