The circadian clock governs many physiological processes, including immune responses which show time of day variations. Here we investigated the impact of time of day on immune responses to acute viral like pathogens using intranasal poly(I:C). We found that the transcriptional profile in the olfactory bulb, a key entry point for viral pathogens, varied significantly depending on the time of challenge. At (Zeitgeber time) ZT12, immune related transcripts were upregulated, indicating enhanced immune surveillance and response to viral infection. Conversely, at ZT0, transcripts associated with inflammation were downregulated, suggesting a dampened immune response. Additionally, we explored the role of acute stress induced corticosterone release in modulating time of day immune responses. Inhibition of corticosterone production with metyrapone altered the expression of clock gene nr1d1 to poly(I:C) in a time-of-day dependent manner, highlighting the interconnection between the circadian clock and acute stress hormone. This study has implications for further understanding of disease states in the context of both circadian and stress biology.