Adult American shad (Alosa sapidissima) were obtained from the Holyoke Dam fish lift, Massachusetts, and transported 68 km by truck to the pool above the Turners Falls Dam. From 1973 to 1976, 6373 shad were transported; average annual mortality was 25%. Mortality of trucked fish was related to river temperature and the number of fish transported at any one time. During trucking a differential mortality occurred between sexes: 2.4 times more females died than males.