This dataset compiles puncture mechanics data from the soft matter and biomechanics literature, aggregating results across a wide range of biological tissues and synthetic hydrogels. For each material, the database records material name/type, probe geometry (tip radius, shape, and angle), sample geometry (width/radius, length, thickness), puncture descriptors (puncture force, puncture depth, etc.), probe velocity, and elastic modulus, along with associated standard deviations where reported. The dataset is intended to support the development and validation of scaling relationships governing soft material puncture, and to serve as a reference for researchers working on the mechanical characterization of soft tissues and hydrogels or related applications.