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Gold Nanoparticle Biodistributions and Stability in Vivo from Mass Spectrometric Imaging

Abstract
Their smaller size, inherent non-toxicity and tunable properties of gold nanoparticles (AuNPs) attract researchers for their use in biological applications such as drug delivery, imaging and therapeutics. Understanding the in vivo fate of these AuNPs are essential for their potential effects in both the environment and the body. In this dissertation, mass spectrometric imaging methods using laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS) and laser desorption/ionization (LDI-MS) have been investigated to monitor the in vivo fate of AuNPs. AuNP injected mouse tissue samples can be obtained and readily imaged to track the injected AuNPs using these methods. A first-ever imaging of 2 nm monolayer protected AuNPs in vivo using LA-ICP-MS is described. In addition, sub-organ biodistribution of AuNPs using LA-ICP-MS has been investigated. An alternative quantification strategy that can be used for LA-ICP-MS is expressed. A dual mode imaging method that can be used to monitor the stability of AuNPs by combining LA-ICP-MS and LDI-MS is reported.
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openaccess
dissertation
Date
2017
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