Abstract Background: Military sexual trauma prevalence has continually increased over the past 25 years. Exposure to sexual trauma, violence, and abuse result in notable health consequences. The Veterans Health Administration has led the integration of universal Military Sexual Trauma (MST) screening and training. Purpose: The purposes of this project were to educate novice nurses and nurse practitioners about MST and to increase their knowledge and comfort in MST screening techniques. Methods: Following an educational session, a pre- and post-study design was used to evaluate improvement in knowledge and comfort screening for MST. Thirty-two participants including PBRNR Nurse Residents, Transition to Practice Nurse Residents, and Nurse Practitioner Residents participated in a 1-hour educational session on military sexual trauma, screening, and its impact on the healthcare setting. Twenty-nine participants fully completed pre and post surveys. These pre and post surveys were used to determine the impact of the educational session on the knowledge, confidence, and opinions of project participants. Results: Nearly 90% eligible nurses participated in the program, and 100% of participants reported that they learned from the educational session. All the participants had less than one year of clinical experience in their roles. Analysis of pre and post survey data found both knowledge and screening for MST increased. There was a significant difference in specific areas of participants MST knowledge after the educational session. Conclusion: The project successfully increased novice nurse and nurse practitioner knowledge and comfort level in screening for MST. Keywords: Clinical reminders, audit and feedback, military sexual trauma, sexual abuse, screening methods, screenings, novice nurse, education