Biochemical Markers and Seedling Characteristics Identify Lupine (Lupinus termis L.) Genotypes


To distinguish among five lupine (Lupinus termis L.) genotypes, biochemical markers and seedling characteristics were studied, using electrophoresis of seed and leaf proteins and four isozyme systems[esterase (EST), catalase (CAT), peroxides (Prx), and glutamate oxaloacetate transaminase (GOT)]. A total of 21 and 13 polymorphic bands were detected in the seed and leaves, respectively. Molecular weights ranged from 183.82 to 11.14 kDa for the seeds and 148.52 to 8.17 for the leaves. Among the genotypes, seed storage protein bands ranged from 10 in genotype Giza-1 to 13 in genotype Giza-3, while the total number of leaf protein bands ranged from six in genotype Giza-2 to nine in genotype Giza-1. Specific, characteristic bands could be used to identify and differentiate some genotypes from among others. At the isozyme level, a similar number of bands were produced, but the location and Rf values of the bands differed, enabling identification among the lupine genotypes.