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ORCID
https://orcid.org/0000-0001-6222-2571
Access Type
Campus-Only Access for Five (5) Years
Document Type
thesis
Degree Program
Chemical Engineering
Degree Type
Master of Science in Chemical Engineering (M.S.Ch.E.)
Year Degree Awarded
2021
Month Degree Awarded
May
Abstract
Hydrogels have been used for many applications, including as a mimic for the extracellular matrix (ECM) in cell culture. For example, a hydrogel containing protease-sensitive substrates can be used to create an environment that cells can modify via enzymatic degradation.
In this study, we propose combining traditional hydrogels for cell culture with “cryptic” site that bury proteolytically cleavable peptide sequences using complex coacervation. Here, the goal is to take advantage of the phase separation of coacervates to protect the cleavable peptide against degradation until acted upon by a mechanical force, such as those generated by adherent cells. To this end, we studied the encapsulation of chymotrypsin as a model protease into our coacervate system and investigated the effect of incorporation into the coacervate on its activity. We have also synthesized a peptide containing cleavable site for both chymotrypsin as our model protease and more biologically relevant matrix metalloproteinases (MMPs). Future efforts will look to incorporate this peptide into both coacervate and hydrogel and test the level of cryptic response.
DOI
https://doi.org/10.7275/23585029.0
First Advisor
Sarah L. Perry
Second Advisor
John Klier
Recommended Citation
Sun, Yimin, "Cryptic Materials And Coacervates" (2021). Masters Theses. 1083.
https://doi.org/10.7275/23585029.0
https://scholarworks.umass.edu/masters_theses_2/1083