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ORCID
https://orcid.org/0000-0002-4157-9894
Access Type
Open Access Thesis
Document Type
thesis
Embargo Period
8-17-2023
Degree Program
Molecular & Cellular Biology
Degree Type
Master of Science (M.S.)
Year Degree Awarded
2023
Month Degree Awarded
September
Abstract
In the summer of 2022, a co-assembly of metagenome was created using the microbes found at Barres Woods in Harvard Forest. 14 samples were taken, and sample was split into the organic and mineral layer, which totals 28 Bulk MAGs. Within this Co-assembly, 4 different genomes were found which were designated with the phylum of FCPU426. Three of which were considered medium quality and one being assigned high quality. The novel phyla first appeared in NCBI and GTDB databases in June 2018. The name FCPU426 dates to 2010 and was named based on the 16s amplicon sequencing.
The novel phylum is shown to have common ancestors with the phyla of Goldbacteria and Firestonebacteria. Goldbacteria was determined to have the ability to break down cellulose to further enhance carbon cycling.
In this analysis of FCPU426, the taxonomy of the phyla will be further investigated. FCPU426 was then annotated to determine if any of the cellulase genes are shared between itself and Goldbacteria. Further annotations were done to spot any genes of interest that create any distinctions between any of its counterparts.
DOI
https://doi.org/10.7275/35954516.0
First Advisor
Jeffery Blanchard
Recommended Citation
Finkelberg, Matthew, "The Discovery of a Novel Bacteria from a Large Co-assembly of Metagenomes" (2023). Masters Theses. 1370.
https://doi.org/10.7275/35954516.0
https://scholarworks.umass.edu/masters_theses_2/1370
Included in
Bacteriology Commons, Computational Biology Commons, Evolution Commons, Forest Biology Commons, Genomics Commons