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ORCID
https://orcid.org/0009-0004-0637-4051
Access Type
Open Access Thesis
Document Type
thesis
Embargo Period
7-2-2023
Degree Program
Chemical Engineering
Degree Type
Master of Science in Chemical Engineering (M.S.Ch.E.)
Year Degree Awarded
2023
Month Degree Awarded
September
Abstract
Blood vessels including the arteries, veins, and capillaries are a critical and indispensable component of various organisms. Some studies estimate that if all the blood vessels present in our body are arranged in line, they would amount to a total length of approximately 60,000 miles. This distance is enough to circle the world two and a half times! In addition to being all pervasive, blood vessels perform certain key functions such as delivery of oxygen and nutrients to various tissues in the body. They also assist in the spread of diseases such as cancer. Therefore, it is important to study vessels from the point of view of tissue engineering applications.
In this study, I have adapted the design of an open-source 3D printed device to create channels in Poly (ethylene glycol) Maleimide (PEG-Mal) hydrogels using the subtractive technique. The PEG-Mal hydrogels can be formed in various formulations to mimic the biophysical and biochemical properties of various tissues such as bone marrow, brain, and lung. These channels created within hydrogels can be easily perfused with physiologically relevant flow rates found in blood vessels and capillaries. Additionally, I have also optimized the hydrogel formulations to improve channel reproducibility. It was found that the number of arms of PEG-Mal contributed the most to channel reproducibility with higher success rates of channel formation in 8-arm gels when compared to 4-arm gels. Therefore, this project delineates the formation of simple in vitro channels in hydrogels which combines properties of the tissue specific extracellular matrix with hemodynamics. It is expected that such a system will find potential use in various tissue engineering and disease modeling studies.
DOI
https://doi.org/10.7275/35791408.0
First Advisor
Shelly R. Peyton
Second Advisor
Peter Beltramo
Third Advisor
Juan Jimenez
Recommended Citation
Kannadasan, Bakthavachalam, "Optimizing Channel Formation in PEG Maleimide Hydrogels" (2023). Masters Theses. 1385.
https://doi.org/10.7275/35791408.0
https://scholarworks.umass.edu/masters_theses_2/1385
Included in
Biochemical and Biomolecular Engineering Commons, Biomedical Engineering and Bioengineering Commons, Polymer Science Commons