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ORCID
N/A
Access Type
Open Access Thesis
Document Type
thesis
Degree Program
Food Science
Degree Type
Master of Science (M.S.)
Year Degree Awarded
2016
Month Degree Awarded
May
Abstract
Hierarchical systems that integrate nano- and macroscale structural elements can offer enhanced stability over traditional immobilization methods. Microparticles were synthesized using interfacial assembly of lipase with (CLMP-N) and without (CLMP) nanoparticles into a crosslinked polymeric core, to determine the impact of the highly ordered system on lipase stability in extreme environments. Kinetic analysis revealed the macrostructure significantly increases the turnover rate (kcat) following immobilization. The macrostructure also stabilized lipase at neutral and basic pH values, while the nanoparticles influenced stability under acidic pH conditions. A greener solvent, choline chloride and urea, was applied to produce sugar ester surfactants. Microparticles exhibited decreases in the turnover rate (kcat) and catalytic efficiency (kcat/Km) following exposure, but retained over 60% and 20% activity after exposure at 50 ºC and 60 ºC, respectively. CLMP and CLMP-N outperformed the commercially available lipase per unit protein in the production of sugar esters. The utilization of greener solvent systems with hierarchical immobilized enzyme systems has the potential to improve processing efficiency and sustainability for the production of value-added agricultural products.
DOI
https://doi.org/10.7275/8420515
First Advisor
Julie Goddard
Second Advisor
Sam Nugen
Third Advisor
Vincent Rotello
Recommended Citation
Andler, Stephanie M., "Influence of Hierarchical Interfacial Assembly on Lipase Stability and Performance in Deep Eutectic Solvent" (2016). Masters Theses. 363.
https://doi.org/10.7275/8420515
https://scholarworks.umass.edu/masters_theses_2/363