Event Title

Metabolomic analysis and variation in phytochemical composition among North American cranberry cultivars

Start Date

29-8-2017 12:00 PM

End Date

29-8-2017 1:15 PM

Description

Abstract.

Cranberries, Vaccinium macrocarpon, are cultivated across several regions of North America. Reported bioactivities of cranberries include antibacterial and antioxidant, with benefits for urinary tract, cardiovascular and gut health. The content of secondary metabolites in the fruit can vary due to factors such as climate, temperature, humidity, cultivar, and disease-related stress; thus a better understanding of how these factors impact composition is desired. Cranberry fruit of multiple cultivars was collected from Massachusetts and Oregon bogs during the 2011 and 2016 growing seasons. Quantitative 1H NMR (qNMR) methods using Assure-RMS software (Bruker Biospin) were developed to quantify triterpenoids and organic acids not easily detected by absorbance-based methods. These include anti-inflammatory compounds ursolic and oleanolic acid, as well as citric, malic, and quinic acids. Using qNMR, cultivar and seasonal differences in these acids were observed. 1H NMR combined with PCA provided non-targeted analysis of variation in fruit composition among samples, revealing similarities and differences between cultivars. Selected polyphenols were also determined using established methods; DMAC assay for total proanthocyanidins (PACs) and HPLC-DAD for quercetin-3-galactoside. As cranberries are a plentiful source of polyphenol antioxidants, a microplate DPPH assay was developed to measure free-radical scavenging antioxidant activity and investigate correlations with fruit composition. The goal is to establish relationships between the health-promoting properties and phytochemical profiles of cranberry fruit from a variety of sources.

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Aug 29th, 12:00 PM Aug 29th, 1:15 PM

Metabolomic analysis and variation in phytochemical composition among North American cranberry cultivars

Abstract.

Cranberries, Vaccinium macrocarpon, are cultivated across several regions of North America. Reported bioactivities of cranberries include antibacterial and antioxidant, with benefits for urinary tract, cardiovascular and gut health. The content of secondary metabolites in the fruit can vary due to factors such as climate, temperature, humidity, cultivar, and disease-related stress; thus a better understanding of how these factors impact composition is desired. Cranberry fruit of multiple cultivars was collected from Massachusetts and Oregon bogs during the 2011 and 2016 growing seasons. Quantitative 1H NMR (qNMR) methods using Assure-RMS software (Bruker Biospin) were developed to quantify triterpenoids and organic acids not easily detected by absorbance-based methods. These include anti-inflammatory compounds ursolic and oleanolic acid, as well as citric, malic, and quinic acids. Using qNMR, cultivar and seasonal differences in these acids were observed. 1H NMR combined with PCA provided non-targeted analysis of variation in fruit composition among samples, revealing similarities and differences between cultivars. Selected polyphenols were also determined using established methods; DMAC assay for total proanthocyanidins (PACs) and HPLC-DAD for quercetin-3-galactoside. As cranberries are a plentiful source of polyphenol antioxidants, a microplate DPPH assay was developed to measure free-radical scavenging antioxidant activity and investigate correlations with fruit composition. The goal is to establish relationships between the health-promoting properties and phytochemical profiles of cranberry fruit from a variety of sources.

This poster is not available for downloading.