Event Title

Identification and mapping of fruit rot resistance QTL in American cranberry using GBS

Start Date

29-8-2017 12:00 PM

End Date

29-8-2017 1:15 PM

Description

Abstract:

Sustainability of the cranberry industry is threatened by widespread and increasing losses due to fruit rot in the field, as well as increasing restrictions on fungicide inputs. Breeding for resistance offers a partial solution, but is challenging because fruit rot is caused by a complex of pathogenic fungi that can vary by location and from year to year. We identified four genetically diverse germplasm accessions that exhibit broad-spectrum fruit rot resistance under field conditions. Three of these accessions were used in biparental crosses to develop four populations segregating for resistance. Genotyping by sequencing was used to generate SNP markers for development of high density genetic maps and QTL analyses. Nineteen QTL associated with fruit rot resistance, distributed on nine linkage groups, were discovered in our populations. Three of these QTL matched previously reported fruit rot resistance QTL. Four newly reported QTL found on linkage group 8 (Vm8), which explain between 21 and 33% of the phenotypic variance for fruit rot, are of particular interest to our breeding program. The populations described herein were also phenotyped for other horticulturally important traits, and QTL associated with yield and berry weight were identified. These QTL provide markers for candidate gene discovery and for future breeding efforts to enhance and pyramid disease resistance and other traits into elite horticultural backgrounds.

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Aug 29th, 12:00 PM Aug 29th, 1:15 PM

Identification and mapping of fruit rot resistance QTL in American cranberry using GBS

Abstract:

Sustainability of the cranberry industry is threatened by widespread and increasing losses due to fruit rot in the field, as well as increasing restrictions on fungicide inputs. Breeding for resistance offers a partial solution, but is challenging because fruit rot is caused by a complex of pathogenic fungi that can vary by location and from year to year. We identified four genetically diverse germplasm accessions that exhibit broad-spectrum fruit rot resistance under field conditions. Three of these accessions were used in biparental crosses to develop four populations segregating for resistance. Genotyping by sequencing was used to generate SNP markers for development of high density genetic maps and QTL analyses. Nineteen QTL associated with fruit rot resistance, distributed on nine linkage groups, were discovered in our populations. Three of these QTL matched previously reported fruit rot resistance QTL. Four newly reported QTL found on linkage group 8 (Vm8), which explain between 21 and 33% of the phenotypic variance for fruit rot, are of particular interest to our breeding program. The populations described herein were also phenotyped for other horticulturally important traits, and QTL associated with yield and berry weight were identified. These QTL provide markers for candidate gene discovery and for future breeding efforts to enhance and pyramid disease resistance and other traits into elite horticultural backgrounds.