Title
Sonodelivery Facilitates Sustained Luciferase Expression from an Episomal Vector in Skeletal Muscle
Publication Date
2015
Journal or Book Title
Materials
Abstract
Successful gene delivery to skeletal muscle is a desirable goal, not only for treating muscle diseases, but also for immunization, treatment of metabolic disorders, and/or delivering gene expression that can treat systemic conditions, such as bone metastatic cancer, for example. Although naked DNA uptake into skeletal muscle is possible, it is largely inefficient in the absence of additional chemical or physical delivery methods. We describe a system for delivery of non-viral or plasmid DNA to skeletal muscle using ultrasound-assisted sonoporation of a nanoplex combining plasmid DNA and a branched polymer based on poly(cyclooctene-graft-oligopeptide). The materials and methods described herein promise to advance the field of sonodelivery and of gene delivery to muscle for therapeutic applications since a simple system is presented that enables long-term gene expression in vivo with the promise of a minimal inflammatory gene expression profile.
DOI
https://doi.org/10.3390/ma8074608
Volume
8
Issue
7
License
UMass Amherst Open Access Policy
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Recommended Citation
Neto, Manoel Figueiredo; Letteri, Rachel; Chan-Seng, Delphine; Emrick, Todd; and Figueiredo, Marxa L., "Sonodelivery Facilitates Sustained Luciferase Expression from an Episomal Vector in Skeletal Muscle" (2015). Materials. 1153.
https://doi.org/10.3390/ma8074608