Publication Date
2021
Journal or Book Title
Scientific Reports
Abstract
Techniques used to clear biological tissue for fluorescence microscopy are essential to connect anatomical principles at levels ranging from subcellular to the whole animal. Here we report a simple and straightforward approach to efficiently render opaque tissue samples transparent and show that this approach can be modified to rapidly label intact tissue samples with antibodies for large volume fluorescence microscopy. This strategy applies a magnetohydrodynamic (MHD) force to accelerate the removal of lipids from tissue samples at least as large as an intact adult mouse brain. We also show that MHD force can be used to accelerate antibody penetration into tissue samples. This strategy complements a growing array of tools that enable high-resolution 3-dimensional anatomical analyses in intact tissues using fluorescence microscopy. MHD-accelerated clearing is simple, fast, reliable, inexpensive, provides good thermal regulation, and is compatible with existing strategies for high-quality fluorescence microscopy of intact tissues.
ISSN
2045-2322
DOI
https://doi.org/10.1038/s41598-021-95692-2
Volume
11
Issue
1
License
UMass Amherst Open Access Policy
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Funder
University of Massachusetts at Amherst; Armstrong Fund for Science; BRAIN [U01-NS108637]; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01MH115094-01A1]; University of Massachusetts at Amherst NSB Fellowship; NSF Postdoctoral Research Fellowship in BiologyNational Science Foundation (NSF) [1812017]
Recommended Citation
Dwyer, Joseph; Ramirez, M Desmond; Katz, Paul S.; Karlstrom, Rolf O.; and Bergan, Joseph, "Accelerated Clearing and Molecular Labeling of Biological Tissues Using" (2021). Scientific Reports. 54.
https://doi.org/10.1038/s41598-021-95692-2