Graphitic Carbon Nitride (C3N4) Reduces Cadmium and Arsenic Phytotoxicity and Accumulation in Rice (Oryza sativa L.)

Authors

Chuanxin Ma, Connecticut Agricultural Experiment StationFollow
yi Hao, University of Massachusetts AmherstFollow
Jian Zhao, Ocean University of ChinaFollow
Nubia Zuverza-Mena, Connecticut Agricultural Experiment StationFollow
Ahmed G. Meselhy, University of Massachusetts AmherstFollow
Om Parkash Dhankher, University of Massachusetts AmherstFollow
Yukui Rui, China Agricultural UniversityFollow
Jason C. White, Connecticut Agricultural Experiment StationFollow
Baoshan Xing, University of Massachusetts - AmherstFollow

Publication Date

2021

Abstract

The present study investigated the role of graphitic carbon nitride (C₃N₄) in alleviating cadmium (Cd)- and arsenic (As)-induced phytotoxicity to rice (Oryza sativa L.). A high-temperature pyrolysis was used to synthesize the C₃N₄, which was characterized by transmission electron microscopy, Fourier-transform infrared spectroscopy, and dynamic light scattering. Rice seedlings were exposed to C₃N₄ at 50 and 250 mg/L in half-strength Hoagland’s solution amended with or without 10 mg/L Cd or As for 14 days. Both Cd and As alone resulted in 26–38% and 49–56% decreases in rice root and shoot biomass, respectively. Exposure to 250 mg/L C₃N₄ alone increased the root and shoot fresh biomass by 17.5% and 25.9%, respectively. Upon coexposure, Cd + C₃N₄ and As + C₃N₄ alleviated the heavy metal-induced phytotoxicity and increased the fresh weight by 26–38% and 49–56%, respectively. Further, the addition of C₃N₄ decreased Cd and As accumulation in the roots by 32% and 25%, respectively, whereas the metal contents in the shoots were 30% lower in the presence of C₃N₄. Both As and Cd also significantly altered the macronutrient (K, P, Ca, S, and Mg) and micronutrient (Cu, Fe, Zn, and Mn) contents in rice, but these alterations were not evident in plants coexposed to C₃N₄. Random amplified polymorphic DNA analysis suggests that Cd significantly altered the genomic DNA of rice roots, while no difference was found in shoots. The presence of C₃N₄ controlled Cd and As uptake in rice by regulating transport-related genes. For example, the relative expression of the Cd transporter OsIRT1 in roots was upregulated by approximately threefold with metal exposure, but C₃N₄ coamendment lowered the expression. Similar results were evident in the expression of the As transporter OsNIP1;1 in roots. Overall, these findings facilitate the understanding of the underlying mechanisms by which carbon-based nanomaterials alleviate contaminant-induced phyto- and genotoxicity and may provide a new strategy for the reduction of heavy metal contamination in agriculture.

Journal or Book Title

Nanomaterials

DOI

https://doi.org/10.3390/nano11040839

Special Issue

The Genetic Changes Induced by Engineered Manufactured Nanomaterials (EMNs)

Volume

11

Issue

4

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.