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Master of Science (M.S.)
Year Degree Awarded
Month Degree Awarded
single molecule spectroscopy
My research has demonstrated the feasibility of the experimental methodologies for proton-sensitive molecular probes of proton transport at the single-molecule level where a single molecule fluorescence spectral shift was observed in poly acrylamide gel (PAAG) matrix after the addition of HCl with a grating spectrometer. Proton-sensitive molecular probes are employed for observing variations of photophysical properties as protons from acid sources transport and react with the reporter molecules such as Coumarin 6 where it has two different protonated states and then shows distinguished emission spectrum. As well, in order to understand the fluorescence properties of neutral and protonated Coumarin 6 in each emission spectrum region, dual-channel detection experiment was built where those fluorescence emission spectrum are allowed to be separated into each optical detection channel. The two-color detection will provide us with the understanding of the heterogeneities indicative of the interaction of pH-responsive probe with its environment. Based on those methodologies and results, this research will lead to investigate how proton transfer kinetics and dynamics are influenced by the geometric arrangements such as inter-functional group spacing, alignment, and flexibility in single macromolecular scaffolds which will suggest the development for future fuel cell and better understanding of biological process.
Michael D Barnes