Off-campus UMass Amherst users: To download campus access dissertations, please use the following link to log into our proxy server with your UMass Amherst user name and password.
Non-UMass Amherst users: Please talk to your librarian about requesting this dissertation through interlibrary loan.
Dissertations that have an embargo placed on them will not be available to anyone until the embargo expires.
ORCID
N/A
Access Type
Open Access Thesis
Document Type
thesis
Degree Program
Food Science
Degree Type
Master of Science (M.S.)
Year Degree Awarded
2016
Month Degree Awarded
February
Abstract
Antimicrobial optimization procedures use the most resistant bacterial culture that could be present in the food to determine the levels of treatment needed to ensure safety. These procedures usually only focus on one method of preparing these inoculums for testing despite prior research showing that the preparation of the culture can influence how the culture reacts to a treatment. In this work, planktonic cells grown in a liquid media and sessile cells grown on a similar solid media were subjected to identical emulsion based antimicrobial systems. The cultures were monitored over time and their numbers periodically enumerated. Weibullian destruction models were used to characterize bacterial death and the different inoculum preparations were separated using ANOVA statistical tests. Using these models highly significant differences between the different sessile and planktonic methods of growth were found. This difference was also found to not be related to the production of curli used in biofilm formation. These results suggest that the methods of inoculum preparation can be a significant factor in bacterial survival, a factor that should be included in food antimicrobial optimization procedures.
DOI
https://doi.org/10.7275/7946914
First Advisor
Lynne A. McLandsborough
Recommended Citation
Murray, Dillon SD, "Influence of Inoculum Preparation upon Sensitivity of Common Food Borne Pathogens to Emulsion Based Antimicrobials" (2016). Masters Theses. 330.
https://doi.org/10.7275/7946914
https://scholarworks.umass.edu/masters_theses_2/330