ScholarWorks@UMassAmherst

Recent Submissions

  • PublicationOpen Access
    LEGO-Inspired Electrically-Actuated Microfluidics for On-Chip Protein Crystallization and In-Situ X-ray Crystallography
    (2026-04) Saha, Sarthak; Chen, Logan; Budziszewski, Gabrielle; Kropek, Sara; Seifert, Kaleb; Cohen, Aina; Russi, Silvia; Bowman, Sarah EJ; Perry, Sarah
    X-ray crystallography has long been the workhorse technique for enabling the analysis and investigation of 3D protein structures. This understanding is crucial for deciphering protein function, including enzymatic reactions, signaling pathways, and more. The initial step in this process involves the crystallization of the target protein. In this pursuit, we have developed a microfluidic device that leverages an electrically-actuated strategy for fluid handling, built on a LEGO-inspired architecture. This device enables on-demand control of counter-diffusive mixing by decoupling reagent loading from mixing, harnessing surface forces without necessitating pumping connections. The LEGO-based architecture involves gold-LEGO-electrodes (GLEs) that are snug fit into a device fabricated by photolithography and nanoimprinting. Our approach entails straightforward pipetting of crystallization reagents into the device to set up counter-diffusion crystallization, followed by the application of <1 V to trigger fluid mixing, thus creating a ‘valve’ that can be easily actuated using AAA batteries, all encompassed into a 150 µm thin device. Fabrication of the device using an X-ray transparent polymer allows for in-situ X-ray crystallography, obviating the need for subsequent extraction and mounting of the protein crystals, and streamlining the process of protein structure determination. Using our LEGO-based electrically-actuated protein crystallization and X-ray crystallography (LEAP-X) platform, we have successfully demonstrated the utility of the device using lysozyme, thaumatin, and proteinase K as model proteins, as well as the crystallization and in-situ, room temperature structural analysis of the metalloprotein rubrerythrin as a novel target. Lastly, we propose the utility of this platform for the addition of chemical triggers for time-resolved protein crystallography.
  • PersonMetadata only
    Lione Mellio
  • PublicationOpen Access
    Developing Design Rules for Polyelectrolyte Complex Materials: Role of Polyelectrolyte Length, Charged Group, and Backbone
    (2026-04) Ramírez Marrero, Isaac André; Ghosh, Ria; Coughlin, Louisa M.; Wong, Wen-Wei; Ng, Emily; Kellner, Elijah; Redder, Madyson; Kaiser, Nadine; von Vacano, Bernhard; Konradi, Rupert; Coughlin, E. Bryan; Perry, Sarah
    Polyelectrolyte complexation is an entropically driven, associative phase separation that has been leveraged to produce aqueously processed plastics known as polyelectrolyte complexes (PECs). Previously, we showed that their affinity to water and their chain mobility are important aspects to consider when designing PEC materials. To establish a more complete picture of influencing parameters, we examined the effect of polymer chemistry, specifically chain length and the side chain and backbone chemistry, on both the phase behavior and mechanical properties of homopolymer PECs. We combined compositional studies of PEC phase behavior with analyses of PEC dynamics and mechanics to understand how these aspects of polymer chemistry affect material performance. We observed that the identity of the ionizable groups heavily affected ion solvation, where PECs with lower water affinities had higher glass transition humidities and were generally more brittle, compared to PECs with higher water affinities. In contrast, backbone chemistry affected chain mobility, allowing acryloyl chemistries to have lower glass transition humidities compared to methacryloyl. Finally, chain length effects depended on the degree of match/mismatch of the polymer’s lengths, with matched PEC systems having higher glass transition humidities than mismatched. Comparisons of the phase behavior and glass transitions revealed that side chain and backbone chemistry effects are universal across different mediums, while length effects are medium specific. These results establish fundamental structure–property relationships for the rational design of functional PEC materials.
  • PublicationOpen Access
    Decentralized Lawfulness: A Conceptual Framework for Emergent Agency
    (2026-04-09) Roohi, Ehsan
    This paper develops Nomological Foundations of Emergent Agency as a conceptual framework for relating micro-level lawfulness, macro-level organization, and emergent human agency within a single layered ontological picture. The proposal is a philosophical framework designed to clarify how locally governed interactions can be conceptually linked to large-scale order and, under suitable organizational conditions, to reason-responsive agency. A central aim of the paper is to prevent category confusion in cross-domain discussions that move too quickly between physics, consciousness, and free will. To this end, the paper introduces a restricted notion of particle-level Computational Description of Dispositional Properties, defined as locally instantiated lawful state-evolution and interaction responsiveness, and explicitly distinguished from phenomenal consciousness. Through a state-space formalization, the paper argues that human agency is more coherently treated as an emergent higher-order capacity involving representation, evaluation, and selfregulation, rather than as a suspension of physical law. The framework is situated within an emergentist-compatibilist interpretation of free will, offering a disciplined interdisciplinary vocabulary for distinguishing local lawfulness, emergent organization, and agential capacities without collapsing them into one another.
  • PublicationOpen Access
    Localized Phase Transitions Controlling Formation and Separation of Hydrogel Interfaces at High Temperatures
    (Royal Society of Chemistry, 2026) Birnbaum, Serena; Crosby, Alfred