Publication Date
2016
Journal or Book Title
Scientific Reports
Abstract
With readily available transcriptome-wide data, understanding the role of each expressed gene is an essential next step. Although RNAi technologies allow for genome-wide screens in cell culture, these approaches cannot replace strategies for discovery in the embryo. Here we present, for the first time, a knockdown screen in mouse preimplantation embryos. Early mammalian development encompasses dynamic cellular, molecular and epigenetic events that are largely conserved from mouse to man. We assayed 712 genes for requirements during preimplantation. We identified 59 genes required for successful development or outgrowth and implantation. We have characterized each phenotype and revealed cellular, molecular, and lineage specific defects following knockdown of transcript. Induced network analyses demonstrate this as a valid approach to identify networks of genes that play important roles during preimplantation. Our approach provides a robust and efficient strategy towards identification of novel phenotypes during mouse preimplantation and facilitates functional annotation of the mammalian transcriptome.
DOI
10.1038/srep37396
Volume
6
License
UMass Amherst Open Access Policy
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Funder
This work was funded in part by March of Dimes Research Grant #6-FY11-367 and NIH R21HD078942 to J.M. W.C. was supported by Lalor Foundation postdoctoral fellowship.
Recommended Citation
Cui, Wei; Dai, Xiangpeng; Marcho, Chelsea; Han, Zhengbin; Zhang, Kun; Tremblay, Kimberly D.; and Mager, Jesse, "Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos" (2016). Scientific Reports. 1.
10.1038/srep37396
Supplementary Table S1
srep37396-s2.xls (49 kB)
Supplementary Table S2
srep37396-s3.pdf (4902 kB)
Supplementary Table S3-S5 and Figure s1-S6