ScholarWorks@UMassAmherst

Recent Submissions

  • PublicationOpen Access
    The Quotidian Web and the Accidental Archive
    (2026) Zuckerman, Ethan; McGrady, Ryan
    Video hosting sites like YouTube are commonly understood through their professional creators and viral content, but they are also rich, global repositories of cultural memory and everyday life. However, their opaque, algorithmically optimized, commercial structure poses challenges to research. We argue that such platforms function as “accidental archives” that capture details of quotidian life that often escape curatorial intention. To consider the unique insights into daily life that such archives preserve, we examine two other accidental archives: a collection of late 20th-century photos and the preserved ruins of Pompeii. We present a four-part mixed-methods approach to researching quotidian video: solving the technical problem of random sampling, conducting metadata analysis, using additional computational means to augment data, and qualitatively analyzing video content.
  • PublicationOpen Access
    One Platform, Four Languages: Comparing English, Spanish, Hindi, and Russian YouTube
    (2025) McGrady, Ryan; Zheng, Kevin; Zuckerman, Ethan
    This study presents a comparative analysis of language-specific random samples of YouTube videos, focusing on English, Spanish, Hindi, and Russian. We produce a large random sample, retrieve metadata, calibrate and deploy language-detection software, and extract four high-confidence language samples. Through an analysis of upload dates, popularity, duration, and category metadata, we highlight patterns and anomalies among our samples. For example, English YouTube has the smallest proportion of videos categorized as “News & Politics,” and Spanish videos have a longer median duration. The most salient contrast, however, is between Hindi YouTube and the other three languages. Hindi videos are much shorter and much newer, with sharp growth since 2020 and more than half of the sample uploaded in 2023 alone. The Hindi sample also exhibits a different pattern of liking, with the lowest percentage of videos with just zero or one like even while it has the highest percentage of videos with just zero or one view. These findings may help to quantify the migration of India’s short-form video culture, based around TikTok, to YouTube when TikTok was banned in the country in 2020. This study underscores the necessity of multilingual and culturally specific approaches to platform research by drawing attention to the heterogeneity of YouTube. We propose this method as a starting point to understand linguistic communities on YouTube, surfacing trends and exceptions while providing cues for more content-focused study.
  • PublicationOpen Access
    LEGO-Inspired Electrically-Actuated Microfluidics for On-Chip Protein Crystallization and In-Situ X-ray Crystallography
    (2026-04) Saha, Sarthak; Chen, Logan; Budziszewski, Gabrielle; Kropek, Sara; Seifert, Kaleb; Cohen, Aina; Russi, Silvia; Bowman, Sarah EJ; Perry, Sarah
    X-ray crystallography has long been the workhorse technique for enabling the analysis and investigation of 3D protein structures. This understanding is crucial for deciphering protein function, including enzymatic reactions, signaling pathways, and more. The initial step in this process involves the crystallization of the target protein. In this pursuit, we have developed a microfluidic device that leverages an electrically-actuated strategy for fluid handling, built on a LEGO-inspired architecture. This device enables on-demand control of counter-diffusive mixing by decoupling reagent loading from mixing, harnessing surface forces without necessitating pumping connections. The LEGO-based architecture involves gold-LEGO-electrodes (GLEs) that are snug fit into a device fabricated by photolithography and nanoimprinting. Our approach entails straightforward pipetting of crystallization reagents into the device to set up counter-diffusion crystallization, followed by the application of <1 V to trigger fluid mixing, thus creating a ‘valve’ that can be easily actuated using AAA batteries, all encompassed into a 150 µm thin device. Fabrication of the device using an X-ray transparent polymer allows for in-situ X-ray crystallography, obviating the need for subsequent extraction and mounting of the protein crystals, and streamlining the process of protein structure determination. Using our LEGO-based electrically-actuated protein crystallization and X-ray crystallography (LEAP-X) platform, we have successfully demonstrated the utility of the device using lysozyme, thaumatin, and proteinase K as model proteins, as well as the crystallization and in-situ, room temperature structural analysis of the metalloprotein rubrerythrin as a novel target. Lastly, we propose the utility of this platform for the addition of chemical triggers for time-resolved protein crystallography.
  • PersonMetadata only
    Lione Mellio
  • PublicationOpen Access
    Developing Design Rules for Polyelectrolyte Complex Materials: Role of Polyelectrolyte Length, Charged Group, and Backbone
    (2026-04) Ramírez Marrero, Isaac André; Ghosh, Ria; Coughlin, Louisa M.; Wong, Wen-Wei; Ng, Emily; Kellner, Elijah; Redder, Madyson; Kaiser, Nadine; von Vacano, Bernhard; Konradi, Rupert; Coughlin, E. Bryan; Perry, Sarah
    Polyelectrolyte complexation is an entropically driven, associative phase separation that has been leveraged to produce aqueously processed plastics known as polyelectrolyte complexes (PECs). Previously, we showed that their affinity to water and their chain mobility are important aspects to consider when designing PEC materials. To establish a more complete picture of influencing parameters, we examined the effect of polymer chemistry, specifically chain length and the side chain and backbone chemistry, on both the phase behavior and mechanical properties of homopolymer PECs. We combined compositional studies of PEC phase behavior with analyses of PEC dynamics and mechanics to understand how these aspects of polymer chemistry affect material performance. We observed that the identity of the ionizable groups heavily affected ion solvation, where PECs with lower water affinities had higher glass transition humidities and were generally more brittle, compared to PECs with higher water affinities. In contrast, backbone chemistry affected chain mobility, allowing acryloyl chemistries to have lower glass transition humidities compared to methacryloyl. Finally, chain length effects depended on the degree of match/mismatch of the polymer’s lengths, with matched PEC systems having higher glass transition humidities than mismatched. Comparisons of the phase behavior and glass transitions revealed that side chain and backbone chemistry effects are universal across different mediums, while length effects are medium specific. These results establish fundamental structure–property relationships for the rational design of functional PEC materials.