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ORCID
N/A
Access Type
Open Access Thesis
Document Type
thesis
Degree Program
Food Science
Degree Type
Master of Science (M.S.)
Year Degree Awarded
2016
Month Degree Awarded
September
Abstract
Probiotics die over time during processing, storage and digestion, resulting in reduced health benefits to the consumer. Microencapsulation of microorganisms is an effective way to improve probiotic viability by restricting cell exposure to extreme conditions through the gastrointestinal tract until release in the colon. In this work, appearance and survival of encapsulated probiotic species from two genera was explored. Lactococcus lactis and Bifidobacterium longum were suspended in calcium alginate microbeads by spraying droplets of alginate-probiotic mixture into calcium chloride solution. This produced uniformly shaped transparent microbeads with high encapsulation yield. Encapsulating Lactococcus lactis extended viability during dry room temperature storage. Encapsulating Bifidobacterium longum revealed high variation between eight different strains from subspecies longum and infantis. Coating alginate particles with chitosan did not improve viability and, viability of free and encapsulated bifidobacteria decreased when exposed to simulated gastric and intestinal conditions. Data from these studies suggest microencapsulating probiotic cells is an invaluable process to extending cell viability. Future research should optimize current formulations to improve encapsulation yield and cell survival during processing, storage, and gastrointestinal transit.
DOI
https://doi.org/10.7275/9052627
First Advisor
David A Sela
Second Advisor
David Julian McClements
Third Advisor
Lynne A McLandsborough
Recommended Citation
Yeung, Timothy W., "Encapsulation of Probiotic Microorganisms in Food-Grade Hydrogel Microbeads for Improving Long-Term Storage and Oral Delivery" (2016). Masters Theses. 459.
https://doi.org/10.7275/9052627
https://scholarworks.umass.edu/masters_theses_2/459