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Open Access Thesis
Master of Science (M.S.)
Year Degree Awarded
Month Degree Awarded
Double strand breaks (DSB) are a common source of DNA damage in both prokaryotes and eukaryotes. If they are not repaired or are repaired incorrectly, they can lead to cell death (bacteria) or cancer (humans). In Escherichia coli, repair of DSB are typically accomplished via homologous recombination and mediated by RecA. This repair pathway, among others, is associated with activation of the SOS response. DNA adenine methyltransferase (dam) mutants have an increased number of DSB and, therefore, are notorious for being RecA-dependent for viability. Here, we show that the synthetic lethality of Δdam/ΔrecA is suppressed when clpP is removed, suggesting that there is a protein, normally degraded by ClpXP, which is preventing DSB from occurring.
M. Sloan Siegrist
Savakis, Amie, "ClpXP-regulated Proteins Suppress Requirement for RecA in Dam Mutants of Escherichia coli K-12" (2018). Masters Theses. 697.