Publication Date
2024
Journal or Book Title
Frontiers in Plant Science
Abstract
Terpenes are important mediators of plant chemical response to environmental cues. Here, we describe the genome-wide identification and biochemical characterization of TPS-a members in Medicago truncatula, a model legume crop. Genome mining identified thirty-nine full-length terpene synthases with a significant number predicted to produce monoterpenes and sesquiterpenes. Biochemical characterization of the TPS-a subfamily associated with sesquiterpene biosynthesis revealed such compounds, that exhibit substantial biological activity in other plants. Gene expression analysis using qPCR and the Medicago gene atlas illustrated distinct tissue and time-based variation in expression in leaves and roots. Together our work establishes the gene-to-metabolite relationships for sesquiterpene synthases in M. truncatula. Understanding the biosynthetic capacity is a foundational step to defining the ecological roles of this important family of compounds.
DOI
https://doi.org/10.3389/fpls.2024.1349009
Volume
15
License
UMass Amherst Open Access Policy
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Recommended Citation
Hendrickson, Hannah; Islam, Monirul; Wabo, Ghislain Fotso; and Mafu, Sibongile, "Biochemical analysis of the TPS-a subfamily in Medicago truncatula" (2024). Frontiers in Plant Science. 28.
https://doi.org/10.3389/fpls.2024.1349009